- Consensus - lists.biobrillouin.org

Consensus Statement (revision to be submitted)
by Kareem Elsayad Jan. 9, 2025

Jan. 9, 2025

Dear All, Firstly, I hope you all had a good start to the new year! We worked through the revision for the consensus statement and would like to send reply to reviewers with amended text by beginning of next week. As mentioned in last email, the reviews were all very nice and straightforward to address. The files can all be found here: https://drive.google.com/drive/folders/1mpQcbs1PCA1XiKrRpwskSeKMCxFXBaZu?us… Please comment on any desired modifications etc. by Monday. Thanks and all the best, Kareem

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Re: FW: [EXTERN] Decision on Nature Photonics manuscript NPHOT-2024-04-00624
by Czarske, Juergen Dec. 17, 2024

Dec. 17, 2024

Great news, Impact 32 David will send comments asap My comments below are only brief THANKS! Cheers Juergen Von: Kareem Elsayad <kareem.elsayad(a)meduniwien.ac.at> Datum: Montag, 16. Dezember 2024 um 04:56 An: "consensus(a)biobrillouin.org" <consensus(a)biobrillouin.org> Betreff: [Consensus Statement] FW: [EXTERN] Decision on Nature Photonics manuscript NPHOT-2024-04-00624 Just got this. Please look through and comment on anything relevant to your particular contribution/expertise (may also poke you individually on this) that we can include in reply. I have no idea who reviewers were but have some guesses. Regardless they are all on our side so should be good: No.1 and No. 2 require a bit of explanation/clarification, and No. 3 is I guess the review you always wished you had 😊. Things are pretty crazy here at end of year (prob by you too!) so I do not want to try and find a date for meeting or the likes - I want to get this out of the way asap. So please comment to this mailing list on any replies to reviewer comments you would like to make. I will then try to integrate them, and we can send the resubmission by end of year. I will post an update to the manuscript with the reviewer reply comments to this mailing list before final resubmission. All the best, Kareem From: <giampaolo.pitruzzello(a)nature.com> Reply to: <giampaolo.pitruzzello(a)nature.com> Date: Monday, 16. December 2024 at 03:57 To: <kareem.elsayad(a)meduniwien.ac.at> Subject: [EXTERN] Decision on Nature Photonics manuscript NPHOT-2024-04-00624 15th December 2024 Dear Kareem, Thank you for your patience. The Consensus statement entitled "Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials" has now been seen by 3 referees, whose comments are attached at the bottom of this email. You will see from their comments that all referees agree on the timeliness, value and broader interest of this Consensus statement. Referees #1 and #2 have raised some seemingly minor concerns that require your attention before we can reach a final decision on the piece. We would accordingly like you to respond to the referees' comments in a revised manuscript, accompanied by a list to explain your revisions. For review purposes, please resubmit the revised manuscript electronically. When you are ready to submit your revised paper, please use the link below (with the changes clearly marked). https://mts-nphot.nature.com/cgi-bin/main.plex?el=A2Bp2BIZ1A1BAYD2J5A9ftdLn… When revising your manuscript please could you: - include a point-by-point response to our referees and a list of any changes you have made to the format of the paper; - ensure it complies with our format requirements as set out in our Guide to Authors at www.nature.com/nphoton/authors/index.html; - note that we will require your article file in an editable format. - state in a cover note the length of the text, methods (if present) and legends; the number of references; the number and estimated final size of figures and tables. Please ensure that all correspondence is marked with your Nature Photonics manuscript reference number in the subject line. Nature Photonics is committed to improving transparency in authorship. As part of our efforts in this direction, we are now requesting that all authors identified as ‘corresponding author’ on published papers create and link their Open Researcher and Contributor Identifier (ORCID) with their account on the Manuscript Tracking System (MTS), prior to acceptance. This applies to primary research papers only. ORCID helps the scientific community achieve unambiguous attribution of all scholarly contributions. You can create and link your ORCID from the home page of the MTS by clicking on ‘Modify my Springer Nature account’. For more information please visit please visit www.springernature.com/orcid. Figshare Nature Portfolio policies strongly encourage you to share your research data in a public repository (e.g. spreadsheets, text, images). We are partnering with the figshare repository to make this easier; you can use the figshare integration via the ‘Research Data Deposition’ tab when submitting your manuscript. Data are stored privately until a manuscript decision is reached. You can edit/withdraw your data up to this point. Your data will be published at the same time as your article. You will receive a data DOI, with guidance on linking your data and manuscript. You retain rights and control over your data. In the event your manuscript is not accepted, you can keep or remove your data in figshare. We recommend the use of discipline-specific repositories where available and for a number of data types this is mandatory. Ensure you do not submit these data types or any sensitive human data to figshare. We look forward to hearing from you soon. Yours sincerely, Dr Giampaolo Pitruzzello Senior Editor Nature Photonics ***************************************************** Reviewers' Comments: ----------------------------- Reviewer #1 (Remarks to the Author): In this Consensus Statement, Bouvet et al aimed to synthesize current knowledge and establish the standard practices to improve the comparability of performance across various implementations of Brillouin light scattering (BLS) microscopy in different laboratories. The authors first outlined the physical quantities measured or derivable from BLS microscopy. They then introduced critical parameters that influence the performance of BLS measurements. Finally, technical details regarding different approaches of BLS microscopy and the common artefacts in practice were presented. The Statement is both timely and critically important for fostering consistent advancements in BLS microscopy. The content is comprehensive, and the conclusions are precise. #Great However, several points need to be addressed before I can recommend this manuscript to the editor for acceptance. Major comments: - The idea of minimum reporting table is excellent, particularly given the challenges of identifying key parameters and comparing performance across published studies. However, I suggest that, in addition to parameters for water or phantom beads, the table or figure captions should include the specific parameters (e.g. laser power, single spectrum acquisition time and pixel step size) used for each biological sample. This inclusion is crucial because parameters often differ between biological samples and phantom materials in practice. #correct, good suggestion - In section Reporting Consensus, (2) spectral resolution (page 5), for frequency-domain spectrometers, I believe the text is referring to the spontaneous Brillouin spectrometer, as elastic scattering spectra cannot be measured using stimulated Brillouin techniques. Regarding the frequency-domain stimulated Brillouin spectrometer, the spectral resolution is theoretically limited by the laser linewidth. Practically, it can also be affected by the numerical aperture (NA) and the scanning speed of the lock-in-amplifier (LIA) relative to its time constant. A more accurate estimate can be derived by deconvolving a measured water spectrum with data obtained under low NA and slow LIA scanning speeds. - In section Reporting Consensus, (3) spectral precision (page 6), the suggestion of ‘0.5 % precision’ for both the ν_B and Γ_B may require reconsideration. For pure water at 780 nm, ν_B is ~5GHz, and Γ_B is ~0.3 GHz, making the ‘0.5 % precision’ equivalent to a shift precision of 25 MHz. This is less stringent than the commonly accepted 10 MHz (refs: Nat. Protoc. 16, 1251–1275 (2021); Biomed. Opt. Express 15, 6024-6035 (2024); and as noted in the 3rd paragraph of page 9). Conversely, a ‘0.5 % precision’ for Γ_B (1.5 MHz) may be impractical, as it would demand prohibitively long averaging times. A precision range in the tens of MHz for linewidth at 780 nm may be more realistic. - In section Calibration spectra (page 7), calibration is only discussed for spontaneous Brillouin scattering. The authors should also include calibration details for stimulated Brillouin scattering. A frequency counter or an electrical spectrum analyzer can be used to measure the beating frequency between pump and probe beams. - In section Laser frequency drifting or line-broadening (page 11), unlike frequency drift, temperature fluctuations rarely cause significant line-broadening in single-longitudinal-mode lasers typically used in BLS microscopy. Since frequency drift is more practically relevant, the authors may consider omitting line-broadening from this discussion. - In Fig. 1E, the large peak Brillouin shift separation observed in the I-SBS results for water and cyclohexane, even exceeding several times the measured linewidth, requires further explanation. The provided reasoning that the I-SBS frequency shift is independent of the refractive index does not sufficiently clarify this observation. - In Fig. 2C and 2D, the Brillouin shift in the central regions of the beads is lower than peripheral regions. Could the authors provide representative spectra for both the central and peripheral regions of the bead? This additional data would help clarify the observed trend. Minor points: - To help reader quickly locate information, specify the section number of the Supplementary Text when referring to it in the main manuscript. - In section Mass density (page 4), measurement of BLS gain in accompany with BLS frequency shift and linewidth may not be sufficient to determine mass density without knowledge of the refractive index. This should be clarified. - Ref. 42 is incorrectly labeled as "in press", despite having been published over four years ago. Please update this citation. Reviewer #2 (Remarks to the Author): The manuscript aims to standardize parameters and concepts in biomedical Brillouin imaging, offering valuable guidance for experts actively working in the field. While it provides a comprehensive consensus, #great several ambiguities require further clarification and elaboration. Additionally, some sections lack clear definitions for the topics under discussion, and in certain instances, the sequence of explanations is inconsistent. The detailed comments are as follows: 1.The paragraphs after the “abstract” and before the “main text” do not have a title. Is it an introduction? 2.It might be beneficial if the authors added a figure of schematics before showing the spectra of different spectrometer designs or in the supplementary material. 3.The introduction lacks a brief discussion about scattering (elastic and inelastic): these terms are used several times later. 4.In “Hypersonic acoustic speed (V)”, what are the acoustic modes? 5.The authors define “Longitudinal Loss Tangent (tan 𝛿)” as a measure of attenuation independent of ⍴. What attenuation? 6.The authors use different notations for shear modulus, G and G´. 7.It might be beneficial to add the definition of acoustic and optical phonon in the introduction as it is used several times later. 8.In “Landau-Placzek Ratio”, “For simple homogeneous samples it is equal to the difference between the specific heat at constant pressure and that at constant volume, relative to that at constant volume.” is hard to understand. 9.In “Resolution,” it would be beneficial to define the spectral resolution before discussing how to improve it by deconvolution or fitting. 10.In “Reporting Consensus”: a.Spectrometer: What is the sampling step size? Is it BS/pixel or something else? b.Spectral resolution: “In the frequency domain, it can be obtained from measuring the elastic scattering peak width from a highly scattering sample like a mirror.” How does this measurement represent the spectral resolution? BS/pixel? c.Spectral resolution: “While the spectral resolution is not a direct indication of the precision with which the key parameters can be extracted if the shape of the peak is known,” known or unknown? Is it a typo? d.Spectral precision: Is this definition applicable to time-varying or heterogeneous samples? It is a systemic parameter that should be characterized for the system, not for samples. 11.The last paragraph of the “Calibration spectra” is a little vague. 12.In the “Fitting of BLS spectra” second paragraph, what variance are the authors referring to? 13.In “Uncertainties,” it is better to define the uncertainty first. Reviewer #3 (Remarks to the Author): Summary and Overall Recommendation: I am pleased to provide a highly positive recommendation for this manuscript. As a generalist in this field, I can appreciate the broader significance of this contribution to the photonics and life sciences community. #great The manuscript addresses a pressing need for standardization in Brillouin Light Scattering (BLS) spectroscopy by proposing a consensus statement for reporting protocols and identifying common artifacts. #great This is a valuable endeavor, given the increasing utilization of BLS in life sciences and the variability in experimental approaches across studies. The participation of leading experts as authors further underscores the importance and reliability of this work. Significance: The field has experienced considerable progress in recent years, but this has been accompanied by challenges, particularly disagreements in the interpretation of measurements and variability in reported results due to differences in apparatus and experimental conditions. By providing clear recommendations for standardized reporting, this consensus statement has the potential to serve as a foundational document that enables reproducibility and consistency in future BLS studies. Moreover, it will help facilitate meaningful comparisons across studies and promote the broader adoption of this technique in biological research. Key Strengths: Timeliness: The manuscript is particularly timely, as BLS studies in biological contexts are still in their nascent stages, often employing custom-built spectrometers. #correct, still research projects also on instrumentation can be got, since further improvements are necessary Establishing agreed-upon standards now will undoubtedly streamline future advancements. Authorship: The inclusion of many leading researchers in this field lends substantial credibility to the work and ensures that the recommendations reflect a broad consensus. #great Practical Utility: The reporting recommendations and detailed discussion of common artifacts are of immense practical value to both new and experienced researchers in this area. #great Clarity: The abstract and overall objectives of the manuscript are clearly articulated, making the motivation and contributions of the study easy to understand. Conclusion: This manuscript makes a highly valuable contribution #great to the field of photonics and its applications in the life sciences. By addressing a long-standing need for standardization in BLS spectroscopy, it is poised to have a significant and lasting impact. I strongly recommend the acceptance of this work without further revisions. #great ***************************************************** This email has been sent through the Springer Nature Tracking System NY-610A-NPG&MTS Confidentiality Statement: This e-mail is confidential and subject to copyright. Any unauthorised use or disclosure of its contents is prohibited. If you have received this email in error please notify our Manuscript Tracking System Helpdesk team at http://platformsupport.nature.com . Details of the confidentiality and pre-publicity policy may be found here http://www.nature.com/authors/policies/confidentiality.html Privacy Policy | Update Profile

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FW: [EXTERN] Decision on Nature Photonics manuscript NPHOT-2024-04-00624
by Kareem Elsayad Dec. 16, 2024

Dec. 16, 2024

Just got this. Please look through and comment on anything relevant to your particular contribution/expertise (may also poke you individually on this) that we can include in reply. I have no idea who reviewers were but have some guesses. Regardless they are all on our side so should be good: No.1 and No. 2 require a bit of explanation/clarification, and No. 3 is I guess the review you always wished you had 😊. Things are pretty crazy here at end of year (prob by you too!) so I do not want to try and find a date for meeting or the likes - I want to get this out of the way asap. So please comment to this mailing list on any replies to reviewer comments you would like to make. I will then try to integrate them, and we can send the resubmission by end of year. I will post an update to the manuscript with the reviewer reply comments to this mailing list before final resubmission. All the best, Kareem From: <giampaolo.pitruzzello(a)nature.com> Reply to: <giampaolo.pitruzzello(a)nature.com> Date: Monday, 16. December 2024 at 03:57 To: <kareem.elsayad(a)meduniwien.ac.at> Subject: [EXTERN] Decision on Nature Photonics manuscript NPHOT-2024-04-00624 15th December 2024 Dear Kareem, Thank you for your patience. The Consensus statement entitled "Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials" has now been seen by 3 referees, whose comments are attached at the bottom of this email. You will see from their comments that all referees agree on the timeliness, value and broader interest of this Consensus statement. Referees #1 and #2 have raised some seemingly minor concerns that require your attention before we can reach a final decision on the piece. We would accordingly like you to respond to the referees' comments in a revised manuscript, accompanied by a list to explain your revisions. For review purposes, please resubmit the revised manuscript electronically. When you are ready to submit your revised paper, please use the link below (with the changes clearly marked). https://mts-nphot.nature.com/cgi-bin/main.plex?el=A2Bp2BIZ1A1BAYD2J5A9ftdLn… When revising your manuscript please could you: - include a point-by-point response to our referees and a list of any changes you have made to the format of the paper; - ensure it complies with our format requirements as set out in our Guide to Authors at www.nature.com/nphoton/authors/index.html; - note that we will require your article file in an editable format. - state in a cover note the length of the text, methods (if present) and legends; the number of references; the number and estimated final size of figures and tables. Please ensure that all correspondence is marked with your Nature Photonics manuscript reference number in the subject line. Nature Photonics is committed to improving transparency in authorship. As part of our efforts in this direction, we are now requesting that all authors identified as ‘corresponding author’ on published papers create and link their Open Researcher and Contributor Identifier (ORCID) with their account on the Manuscript Tracking System (MTS), prior to acceptance. This applies to primary research papers only. ORCID helps the scientific community achieve unambiguous attribution of all scholarly contributions. You can create and link your ORCID from the home page of the MTS by clicking on ‘Modify my Springer Nature account’. For more information please visit please visit www.springernature.com/orcid. Figshare Nature Portfolio policies strongly encourage you to share your research data in a public repository (e.g. spreadsheets, text, images). We are partnering with the figshare repository to make this easier; you can use the figshare integration via the ‘Research Data Deposition’ tab when submitting your manuscript. Data are stored privately until a manuscript decision is reached. You can edit/withdraw your data up to this point. Your data will be published at the same time as your article. You will receive a data DOI, with guidance on linking your data and manuscript. You retain rights and control over your data. In the event your manuscript is not accepted, you can keep or remove your data in figshare. We recommend the use of discipline-specific repositories where available and for a number of data types this is mandatory. Ensure you do not submit these data types or any sensitive human data to figshare. We look forward to hearing from you soon. Yours sincerely, Dr Giampaolo Pitruzzello Senior Editor Nature Photonics ***************************************************** Reviewers' Comments: ----------------------------- Reviewer #1 (Remarks to the Author): In this Consensus Statement, Bouvet et al aimed to synthesize current knowledge and establish the standard practices to improve the comparability of performance across various implementations of Brillouin light scattering (BLS) microscopy in different laboratories. The authors first outlined the physical quantities measured or derivable from BLS microscopy. They then introduced critical parameters that influence the performance of BLS measurements. Finally, technical details regarding different approaches of BLS microscopy and the common artefacts in practice were presented. The Statement is both timely and critically important for fostering consistent advancements in BLS microscopy. The content is comprehensive, and the conclusions are precise. However, several points need to be addressed before I can recommend this manuscript to the editor for acceptance. Major comments: - The idea of minimum reporting table is excellent, particularly given the challenges of identifying key parameters and comparing performance across published studies. However, I suggest that, in addition to parameters for water or phantom beads, the table or figure captions should include the specific parameters (e.g. laser power, single spectrum acquisition time and pixel step size) used for each biological sample. This inclusion is crucial because parameters often differ between biological samples and phantom materials in practice. - In section Reporting Consensus, (2) spectral resolution (page 5), for frequency-domain spectrometers, I believe the text is referring to the spontaneous Brillouin spectrometer, as elastic scattering spectra cannot be measured using stimulated Brillouin techniques. Regarding the frequency-domain stimulated Brillouin spectrometer, the spectral resolution is theoretically limited by the laser linewidth. Practically, it can also be affected by the numerical aperture (NA) and the scanning speed of the lock-in-amplifier (LIA) relative to its time constant. A more accurate estimate can be derived by deconvolving a measured water spectrum with data obtained under low NA and slow LIA scanning speeds. - In section Reporting Consensus, (3) spectral precision (page 6), the suggestion of ‘0.5 % precision’ for both the ν_B and Γ_B may require reconsideration. For pure water at 780 nm, ν_B is ~5GHz, and Γ_B is ~0.3 GHz, making the ‘0.5 % precision’ equivalent to a shift precision of 25 MHz. This is less stringent than the commonly accepted 10 MHz (refs: Nat. Protoc. 16, 1251–1275 (2021); Biomed. Opt. Express 15, 6024-6035 (2024); and as noted in the 3rd paragraph of page 9). Conversely, a ‘0.5 % precision’ for Γ_B (1.5 MHz) may be impractical, as it would demand prohibitively long averaging times. A precision range in the tens of MHz for linewidth at 780 nm may be more realistic. - In section Calibration spectra (page 7), calibration is only discussed for spontaneous Brillouin scattering. The authors should also include calibration details for stimulated Brillouin scattering. A frequency counter or an electrical spectrum analyzer can be used to measure the beating frequency between pump and probe beams. - In section Laser frequency drifting or line-broadening (page 11), unlike frequency drift, temperature fluctuations rarely cause significant line-broadening in single-longitudinal-mode lasers typically used in BLS microscopy. Since frequency drift is more practically relevant, the authors may consider omitting line-broadening from this discussion. - In Fig. 1E, the large peak Brillouin shift separation observed in the I-SBS results for water and cyclohexane, even exceeding several times the measured linewidth, requires further explanation. The provided reasoning that the I-SBS frequency shift is independent of the refractive index does not sufficiently clarify this observation. - In Fig. 2C and 2D, the Brillouin shift in the central regions of the beads is lower than peripheral regions. Could the authors provide representative spectra for both the central and peripheral regions of the bead? This additional data would help clarify the observed trend. Minor points: - To help reader quickly locate information, specify the section number of the Supplementary Text when referring to it in the main manuscript. - In section Mass density (page 4), measurement of BLS gain in accompany with BLS frequency shift and linewidth may not be sufficient to determine mass density without knowledge of the refractive index. This should be clarified. - Ref. 42 is incorrectly labeled as "in press", despite having been published over four years ago. Please update this citation. Reviewer #2 (Remarks to the Author): The manuscript aims to standardize parameters and concepts in biomedical Brillouin imaging, offering valuable guidance for experts actively working in the field. While it provides a comprehensive consensus, several ambiguities require further clarification and elaboration. Additionally, some sections lack clear definitions for the topics under discussion, and in certain instances, the sequence of explanations is inconsistent. The detailed comments are as follows: 1.The paragraphs after the “abstract” and before the “main text” do not have a title. Is it an introduction? 2.It might be beneficial if the authors added a figure of schematics before showing the spectra of different spectrometer designs or in the supplementary material. 3.The introduction lacks a brief discussion about scattering (elastic and inelastic): these terms are used several times later. 4.In “Hypersonic acoustic speed (V)”, what are the acoustic modes? 5.The authors define “Longitudinal Loss Tangent (tan 𝛿)” as a measure of attenuation independent of ⍴. What attenuation? 6.The authors use different notations for shear modulus, G and G´. 7.It might be beneficial to add the definition of acoustic and optical phonon in the introduction as it is used several times later. 8.In “Landau-Placzek Ratio”, “For simple homogeneous samples it is equal to the difference between the specific heat at constant pressure and that at constant volume, relative to that at constant volume.” is hard to understand. 9.In “Resolution,” it would be beneficial to define the spectral resolution before discussing how to improve it by deconvolution or fitting. 10.In “Reporting Consensus”: a.Spectrometer: What is the sampling step size? Is it BS/pixel or something else? b.Spectral resolution: “In the frequency domain, it can be obtained from measuring the elastic scattering peak width from a highly scattering sample like a mirror.” How does this measurement represent the spectral resolution? BS/pixel? c.Spectral resolution: “While the spectral resolution is not a direct indication of the precision with which the key parameters can be extracted if the shape of the peak is known,” known or unknown? Is it a typo? d.Spectral precision: Is this definition applicable to time-varying or heterogeneous samples? It is a systemic parameter that should be characterized for the system, not for samples. 11.The last paragraph of the “Calibration spectra” is a little vague. 12.In the “Fitting of BLS spectra” second paragraph, what variance are the authors referring to? 13.In “Uncertainties,” it is better to define the uncertainty first. Reviewer #3 (Remarks to the Author): Summary and Overall Recommendation: I am pleased to provide a highly positive recommendation for this manuscript. As a generalist in this field, I can appreciate the broader significance of this contribution to the photonics and life sciences community. The manuscript addresses a pressing need for standardization in Brillouin Light Scattering (BLS) spectroscopy by proposing a consensus statement for reporting protocols and identifying common artifacts. This is a valuable endeavor, given the increasing utilization of BLS in life sciences and the variability in experimental approaches across studies. The participation of leading experts as authors further underscores the importance and reliability of this work. Significance: The field has experienced considerable progress in recent years, but this has been accompanied by challenges, particularly disagreements in the interpretation of measurements and variability in reported results due to differences in apparatus and experimental conditions. By providing clear recommendations for standardized reporting, this consensus statement has the potential to serve as a foundational document that enables reproducibility and consistency in future BLS studies. Moreover, it will help facilitate meaningful comparisons across studies and promote the broader adoption of this technique in biological research. Key Strengths: Timeliness: The manuscript is particularly timely, as BLS studies in biological contexts are still in their nascent stages, often employing custom-built spectrometers. Establishing agreed-upon standards now will undoubtedly streamline future advancements. Authorship: The inclusion of many leading researchers in this field lends substantial credibility to the work and ensures that the recommendations reflect a broad consensus. Practical Utility: The reporting recommendations and detailed discussion of common artifacts are of immense practical value to both new and experienced researchers in this area. Clarity: The abstract and overall objectives of the manuscript are clearly articulated, making the motivation and contributions of the study easy to understand. Conclusion: This manuscript makes a highly valuable contribution to the field of photonics and its applications in the life sciences. By addressing a long-standing need for standardization in BLS spectroscopy, it is poised to have a significant and lasting impact. I strongly recommend the acceptance of this work without further revisions. ***************************************************** This email has been sent through the Springer Nature Tracking System NY-610A-NPG&MTS Confidentiality Statement: This e-mail is confidential and subject to copyright. Any unauthorised use or disclosure of its contents is prohibited. If you have received this email in error please notify our Manuscript Tracking System Helpdesk team at http://platformsupport.nature.com . Details of the confidentiality and pre-publicity policy may be found here http://www.nature.com/authors/policies/confidentiality.html Privacy Policy | Update Profile

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FW: [EXTERN] arXiv New submission -> 2411.11712 in physics.optics from Kareem.elsayad@meduniwien.ac.at
by Kareem Elsayad Nov. 19, 2024

Nov. 19, 2024

On 19.11.24, 04:00, "e-prints(a)arxiv.org <mailto:e-prints@arxiv.org>" <e-prints(a)arxiv.org <mailto:e-prints@arxiv.org>> wrote: Your submission submit/6002321 has been assigned the permanent arXiv identifier 2411.11712 and is available at: http://arxiv.org/abs/2411.11712 <http://arxiv.org/abs/2411.11712> The paper password for this article is: peexv Please share this with your co-authors. They may use it to claim ownership. Abstract will appear in today's mailing as: ------------------------------------------------------------------------------ \\ arXiv:2411.11712 From: Kareem Elsayad <Kareem.elsayad(a)meduniwien.ac.at <mailto:Kareem.elsayad@meduniwien.ac.at>> Date: Mon, 18 Nov 2024 16:37:33 GMT (2451kb) Title: Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials Authors: Pierre Bouvet, Carlo Bevilacqua, Yogeshwari Ambekar, Giuseppe Antonacci, Joshua Au, Silvia Caponi, Sophie Chagnon-Lessard, Juergen Czarske, Thomas Dehoux, Daniele Fioretto, Yujian Fu, Jochen Guck, Thorsten Hamann, Dag Heinemann, Torsten J\"ahnke, Hubert Jean-Ruel, Irina Kabakova, Kristie Koski, Nektarios Koukourakis, David Krause, Salvatore La Cavera III, Timm Landes, Jinhao Li, Jeremie Margueritat, Maurizio Mattarelli, Michael Monaghan, Darryl R. Overby, Fernando Perez-Cota, Emanuele Pontecorvo, Robert Prevedel, Giancarlo Ruocco, John Sandercock, Giuliano Scarcelli, Filippo Scarponi, Claudia Testi, Peter T\"or\"ok, Lucie Vovard, Wolfgang Weninger, Vladislav Yakovlev, Seok-Hyun Yun, Jitao Zhang, Francesca Palombo, Alberto Bilenca, Kareem Elsayad Categories: physics.optics physics.bio-ph physics.ins-det Comments: Main Text & Supplementary Text: 56 pages, 3 Figures, 2 Supplementary Figures, 1 Supplementary Table License: http://creativecommons.org/licenses/by/4.0/ <http://creativecommons.org/licenses/by/4.0/> \\ Brillouin Light Scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the sub-micron scale. Over the last decade it has seen increased applications in the life sciences, driven by the observed significance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and its extension to an imaging modality. As with other spectroscopic techniques, BLS measurements not only detect signals characteristic of the investigated sample, but also of the experimental apparatus, and can be significantly affected by measurement conditions. The aim of this consensus statement is to improve the comparability of BLS studies by providing reporting recommendations for the measured parameters and detailing common artifacts. Given that most BLS studies of biological matter are still at proof-of-concept stages and use different--often self-built--spectrometers, a consensus statement is particularly timely to assure unified advancement. \\ Contains: CONSENSUS_ArXiv_v2.pdf: 2510309 bytes

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consensus manuscript on arXiv
by Kareem Elsayad Nov. 16, 2024

Nov. 16, 2024

Dear All, Per request from several of you we have uploaded the manuscript to the arXiv preprint server. Due to the formatting constraints of arXiv (which makes it difficult to have supp mat) we have combined supp text with the main text and put other supp mat on figshare. I took this opportunity to also make some edits people sent me after submission (most notably grammar corrections, the affiliations of some of you, and indexing and titles in supp text to make more readable/coherent). I uploaded this (arXiv) submitted version here: https://drive.google.com/file/d/1q194xOFTzMG9VF96M8J0dre4_dRl-aUG/view?usp=… (.pdf version) https://docs.google.com/document/d/1SyLc7Ef7eM7N4Hd_j11cO3DVmnwisAYN/edit?u… (.docx version) arXiv just wrote me the following.. Your article is currently scheduled to be announced at Tue, 19 Nov 2024 01:00:00 GMT. Updates before Mon, 18 Nov 2024 19:00:00 GMT will not delay announcement. As such, in case you would still like any (minor) changes please let me know asap and very latest by Monday 18th Nov. 15:00 GMT Thanks and all the best, Kareem

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Consensus statement (submitted)
by Kareem Elsayad Nov. 13, 2024

Nov. 13, 2024

Dear All, We finally submitted the manuscript earlier this afternoon (I have yet to receive any confirmation email – which is a bit strange, but online it says submitted and editor assigned). The final manuscript files (Main Text and Supplementary Text) can be found here: https://drive.google.com/file/d/1rGMNPmpbKGXNSCoa6obnj_ZyrhTy3JGM/view?usp=… https://drive.google.com/file/d/1y70F3Rf44Legs85xM-74UDr7Pgh5DEuS/view?usp=… (also submitted were the reporting tables in zipped format, and template thereof) I hope we managed to respect/implement your edits/suggestions.. In some cases, compromises had to be made -- but I don’t think these were major. Eitherway, we should have another round to discuss things when we get it back. About author order. I was checking through what convention is in consensus statements. In the end, I have put Pierre Bouvet and Carlo Bevilacqua up front since they really in my opinion did an exceptional amount of work behind the scenes in making manuscript what it is. I then listed everybody in alphabetic order. I understand some contributed more than others, but please understand we cannot rank contributions of >40 people without going insane (and making someone unhappy). At the end I tack Francesca, Alberto and myself as this was initially planned by us in communication with the editor. I have seen this type of author ordering applied previously in numerous consensus statements (such as recent ones in Nature Energy and Nature Medicine). Less frequently used alternatives are everybody in alphabetic order (more usual for very large consortia), or ranking in terms of contribution (more usual for smaller author lists). I hope this is acceptable for everyone. If anyone has issues with this though let me know and I am ofcourse happy to discuss on amending for resubmission. In the end it was I think a great group effort (if at times exhausting – it took me forever to enter all your affiliations & info online!). I wanted to thank you all again for your valuable contributions and time - I think we have something quite important that we can all be proud of! All the best (& I’ll keep you posted with any news on this front), Kareem

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Re: Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials
by Kareem Elsayad Nov. 8, 2024

Nov. 8, 2024

Dear All, Thank you for going through the manuscript and providing corrections/suggestions. We have already started addressing a number of these. From tomorrow noon I will take the manuscript offline to implement these properly with references and modified figures. If you have not yet entered your affiliation and email please do not forget to do so before Monday morning in the excel doc. Here is the link again: https://docs.google.com/spreadsheets/d/1YP-5kgcZcC3FViogO44GN-vm7JDSmjZk/ed… Also IMPORTANTLY, I completely forgot, I will also need a statement if you have any conflicts of interests. I created an extra column in the above excel file to enter if you do. Please enter a full sentence explaining any conflicts of interest you think relevant to report. If you do not have any, then no action is needed here. The specific statement on the submission pages reads as follows: the authors have competing interests as defined by Nature Portfolio, or other interests that might be perceived to influence the results and/or discussion reported in this paper. These interests are listed in a paragraph at the end of the manuscript. You can find a list of what Nature consider constitutes conflicts of interests here: https://www.nature.com/nature-portfolio/editorial-policies/competing-intere… I paste the relevant section from this webpage at the bottom of this message. Also do not forget to send me any updated reporting tables by Sunday. Very finally, I received a lot of requests to include additional references to people’s work (both per email and as comments in document). It may not be possible to include all due to space. We will prioritize the ones that are needed to illustrate/justify an already discussed point and relevant to the concept of journal/article and its expected readership… ….while, when possible, trying to respect contributions from different labs. I ask for your understanding in advance though, that we likely cannot include every suggested reference. If you have any important comments, please feel free to write ofcourse. I may spontaneously contact some of you over the weekend if there are any burning questions. But I think we are good for the most part, and you will probably hear next from me when we submitted on Monday! All the best, Kareem CONFLICTS OF INTEREST: In the interests of transparency and to help readers form their own judgements of potential bias, Nature Portfolio journals' require authors to declare any competing financial and/or non-financial interests in relation to the work described. The corresponding author is responsible for submitting a competing interests' statement on behalf of all authors of the paper. Definition For the purposes of this policy, competing interests are defined as financial and non-financial interests that could directly undermine, or be perceived to undermine the objectivity, integrity and value of a publication, through a potential influence on the judgements and actions of authors with regard to objective data presentation, analysis and interpretation. Financial competing interests include any of the following: Funding: Research support (including salaries, equipment, supplies, and other expenses) by organizations that may gain or lose financially through this publication. A specific role for the funder in the conceptualization, design, data collection, analysis, decision to publish, or preparation of the manuscript, should be disclosed. Employment: Recent (while engaged in the research project), present or anticipated employment by any organization that may gain or lose financially through this publication. Personal financial interests: Stocks or shares in companies that may gain or lose financially through publication; consultation fees or other forms of remuneration (including reimbursements for attending symposia) from organizations that may gain or lose financially; patents or patent applications (awarded or pending) filed by the authors or their institutions whose value may be affected by publication. For patents and patent applications, disclosure of the following information is requested: patent applicant (whether author or institution), name of inventor(s), application number, status of application, specific aspect of manuscript covered in patent application. It is difficult to specify a threshold at which a financial interest become significant, but note that many US universities require faculty members to disclose interests exceeding $10,000 or 5% equity in a company (see, for example, B. Lo et al. New Engl. J. Med. 343, 1616-1620; 2000). Any such figure is necessarily arbitrary, so we offer as one possible practical alternative guideline: "Any undeclared competing financial interests that could embarrass you were they to become publicly known after your work was published." We do not consider diversified mutual funds or investment trusts to constitute a competing financial interest. Non-financial competing interests: Non-financial competing interests can take different forms, including personal or professional relations with organizations and individuals. We would encourage authors and referees to declare any unpaid roles or relationships that might have a bearing on the publication process. Examples of non-financial competing interests include (but are not limited to): Unpaid membership in a government or non-governmental organization Unpaid membership in an advocacy or lobbying organization Unpaid advisory position in a commercial organization Writing or consulting for an educational company Acting as an expert witness From: Kareem Elsayad <kareem.elsayad(a)meduniwien.ac.at> Date: Tuesday, 5. November 2024 at 12:29 To: "consensus(a)biobrillouin.org" <consensus(a)biobrillouin.org> Subject: Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials Dear All, You are receiving this email as you are part of the author list of the BLS consensus statement we are now finalizing for Nature Photonics. We have finally managed to cut the manuscript down (by a factor of >5) to fit into the required format and tried our best to make it into a somewhat coherent story, while accommodating most requests. We would like to submit it on Monday next week. Please take a read through the Manuscript (Main Text) and Supplementary Text here and add any comments you see relevant. When doing so, remember we are tight on space, and we can have at times up to almost 40 different opinions (approx. number of authors), but we will do our best to accommodate. It would be really great if you could provide your comments before the weekend so we can implement course of Sat & Sun. Here are the main files, which you should also have access to edit: Main Text: https://docs.google.com/document/d/1mdSDVePGOB8y0B4mCZpiKr_pFvF5WDeZ/edit?u… Supplementary Text: https://docs.google.com/document/d/1d5wA-QFlYJNiVtgA20ENMZnhJUSRzjO5/edit?u… Also, for the submission we will have to enter all your affiliations and any requested mentions of funding sources, etc. To not write everyone individually, could you please be sure to fill in the following online excel sheet before Monday: Affiliation and funding .xlsx file: https://docs.google.com/spreadsheets/d/1YP-5kgcZcC3FViogO44GN-vm7JDSmjZk/ed… If you submitted any data (and especially if you see your image in the manuscript) please double check the completeness of the Minimum Reporting Table you submitted with the data, and as needed send me an updated version. These will be provided with the manuscript as Supplementary Data. Neither we (nor the journal) will edit what you entered further, so please make sure it is as you would like to see it in print online. (FYI the Minimum Reporting Table file is still available here: https://docs.google.com/spreadsheets/d/1i-bz6QJ0l3LZl8YUgCw7b3hkzdWrtW_v/ed…) Last but not least, since there are so many authors (and I cannot send to all at once in cc/bcc using my University account – it thinks I have been hacked!), I created this mailing list consensus(a)biobrillouin.org If you would like to write all the authors you may also post to this, but please do not send files to this mailing list or everybody gets them (and it will clog everyones mailbox). If you share any files, the best option is to upload here: https://drive.google.com/drive/folders/1zBkQajxKTgjzUju19fdrFPFZC1r5O5Kq?us… and send me a quick email stating you have uploaded something and what it is you have uploaded. All the best, Kareem

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Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials
by Kareem Elsayad Nov. 5, 2024

Nov. 5, 2024

Dear All, You are receiving this email as you are part of the author list of the BLS consensus statement we are now finalizing for Nature Photonics. We have finally managed to cut the manuscript down (by a factor of >5) to fit into the required format and tried our best to make it into a somewhat coherent story, while accommodating most requests. We would like to submit it on Monday next week. Please take a read through the Manuscript (Main Text) and Supplementary Text here and add any comments you see relevant. When doing so, remember we are tight on space, and we can have at times up to almost 40 different opinions (approx. number of authors), but we will do our best to accommodate. It would be really great if you could provide your comments before the weekend so we can implement course of Sat & Sun. Here are the main files, which you should also have access to edit: Main Text: https://docs.google.com/document/d/1mdSDVePGOB8y0B4mCZpiKr_pFvF5WDeZ/edit?u… Supplementary Text: https://docs.google.com/document/d/1d5wA-QFlYJNiVtgA20ENMZnhJUSRzjO5/edit?u… Also, for the submission we will have to enter all your affiliations and any requested mentions of funding sources, etc. To not write everyone individually, could you please be sure to fill in the following online excel sheet before Monday: Affiliation and funding .xlsx file: https://docs.google.com/spreadsheets/d/1YP-5kgcZcC3FViogO44GN-vm7JDSmjZk/ed… If you submitted any data (and especially if you see your image in the manuscript) please double check the completeness of the Minimum Reporting Table you submitted with the data, and as needed send me an updated version. These will be provided with the manuscript as Supplementary Data. Neither we (nor the journal) will edit what you entered further, so please make sure it is as you would like to see it in print online. (FYI the Minimum Reporting Table file is still available here: https://docs.google.com/spreadsheets/d/1i-bz6QJ0l3LZl8YUgCw7b3hkzdWrtW_v/ed…) Last but not least, since there are so many authors (and I cannot send to all at once in cc/bcc using my University account – it thinks I have been hacked!), I created this mailing list consensus(a)biobrillouin.org If you would like to write all the authors you may also post to this, but please do not send files to this mailing list or everybody gets them (and it will clog everyones mailbox). If you share any files, the best option is to upload here: https://drive.google.com/drive/folders/1zBkQajxKTgjzUju19fdrFPFZC1r5O5Kq?us… and send me a quick email stating you have uploaded something and what it is you have uploaded. All the best, Kareem

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Test 7 MUW
by Kareem Elsayad Oct. 19, 2024

Oct. 19, 2024

Test 7 from MUW

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Gmail check 6
by Kareem Elsayad Oct. 19, 2024

Oct. 19, 2024

Check 6 from gmail

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